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IP Archives of Cytology and Histopathology Research


Liver biopsy evaluation in chronic hepatitis b infection: ‘Back to basics’ - re-visiting semi-quantitative scoring systems


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Author Details: Aneeta Jassar*,V K Kumar

Volume : 3

Issue : 3

Online ISSN : 2456-9267

Print ISSN : 2581-5725

Article First Page : 124

Article End Page : 130


Abstract

Context: Despite advances in understanding into the pathophysiology of chronic hepatitis B, liver biopsy evaluation on H&E stained sections has stood the test of time. There is therefore, a need to re-visit the most commonly used scoring systems with an aim to look for an ideal semi-quantitative system which is objective, simple to apply and versatile enough to incorporate newer advances.
Aims: To compare various semi-quantitative scoring systems with a view to (a) look for consistency in reporting, (b) assess usefulness in follow-up and (c) recommend a scoring system that is practical and easy to apply.
Settings and Design: Chronic hepatitis B was defined as HBsAg positive status for at least six months. All liver biopsies were evaluated using semi-quantitative scoring systems viz the Knodell, Ishak’s modified HAI and Scheuer systems.
Materials and Methods: Baseline biopsies were assessed for intra- and inter-observer variation in order to find the system best suited for routine use. The observer variation was also calculated separately for portal, peri-portal and lobular inflammation as well as for fibrosis.
Statistical Analysis used: A Kappa analysis up to 95% confidence interval was done to look for statistical significance of inter- and intra-observer agreement between the three scoring systems.
Results: On multivariate Kappa analysis, it was found that both inter- and intra-observer agreement were comparable in the Knodell and Ishak systems, and was significantly better with the Scheuer system. Further, agreement was better with fibrosis than with necro-inflammatory scores.
Conclusions: The authors recommend Ishak’s modified system as it conveyed the most relevant and exhaustive information.

Keywords: Chronic hepatitis B, Ishak’s system, Knodell’s system, Liver biopsy, Scheuer system, Semi-quantitative scoring systems.

Doi :-https://doi.org/10.18231/2456-9267.2018.0026