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Indian Journal of Clinical Anatomy and Physiology


Micronucleus assay in formalin exposed individuals


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Author Details: N. Pratheepa Sivasankari, S. Sundarapandian, E Sakthivel

Volume : 5

Issue : 1

Online ISSN : 2394-2126

Print ISSN : 2394-2118

Article First Page : 81

Article End Page : 84


Abstract

Introduction: The aim of the study was to find out the nuclear abnormalities like Micronuclei in the oral mucosa of the formalin exposed individuals in the department of anatomy. This MN Aaasy is an inexpensive method.
Materials and Methods: Faculties and staffs working in the department of anatomy and the first-year students were included in this study. Study sample consists of 50 subjects and was divided into 5 groups according the years of exposure. By scraping with the wooden spatula in the buccal mucosa Oral squamous cells were collected and smeared on the slides. The smeared slides were fixed with methanol glutaraldehyde fixative and stained with giemsa, maygrunwald stain. After airdrying 1000 cells were screened for Micronucleus (MN).
Results: Micronuclei frequency was more in the group who had more than 15 years of formalin exposure. The mean MN count was 9.60 and it was statistically significant when compared to other groups.
Conclusion: Micronucleus Assay is the most simple, feasible, inexpensive method to find out the nuclear abnormalities at an early stage in the formalin exposed individuals.

Keywords: Formalin, Buccal Mucosa, Micronucleus.

Doi :-https://doi.org/10.18231/2394-2126.2018.0019