International Journal of Clinical Biochemistry and Research


Serum levels of SOD, MDA and glutathione peroxidase in different subtypes of cataract patients


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Author Details: Jaskiran Kaur, Anju Sharma, Sahiba Kukreja, Neha, Amandeep Kaur, Mandeep Kaur

Volume : 3

Issue : 3

Online ISSN : 2394-6377

Print ISSN : 2394-6369

Article First Page : 267

Article End Page : 271


Abstract

Background: Oxidative stress is essentially an imbalance between the production of free radicals and the ability of the body to counteract or detoxify their harmful effects through neutralization by antioxidants. Since the reactive oxygen species (ROS) generated from multiple sources is capable of producing deleterious effects. Body has its own defence mechanism against the oxidative damage by ROS. These defence strategies act in the form of Antioxidants.
Methods: The present study was carried out in the Department of Biochemistry, Govt. Medical College, Amritsar in collaboration with Department of Ophthalmology, Ram Lal Eye Hospital, Amritsar attached to Govt. Medical College, Amritsar. 50 patients suffering from cataract attending the OPD and wards of Ramlal Eye Hospital diagnosed on the basis of history, clinical signs/symptoms and slit lamp examination were selected for the study. 50 age matched normal healthy individuals from the same population were selected to serve as controls. The comparison was done by students ‘t’ test on the number of variables for each parameter.
Result: Our results confirm that oxidative stress is responsible for the development of all three subtypes of cataract. The use of antioxidants may be helpful to prevent or delay cataractogenesis.
Conclusion: We found that there is a significant disequilibrium status of antioxidant system in serum in various types of age- related cataract patients compared with the control group. The activities of SOD, GPx in cataract group were lower than those in the control group. The oxidative stress products MDA were significantly increased in serum of cataract patients.

Key words:
Oxidative stress, Cortical cataract, Posterior sub- capsular, Nuclear cataract