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International Journal of Clinical Biochemistry and Research

Elimination of bilirubin interference by photolysis in the analysis of creatinine, glucose and alkaline phosphatase

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Author Details: PM Abraham Sam Rajan

Volume : 4

Issue : 1

Online ISSN : 2394-6377

Print ISSN : 2394-6369

Article First Page : 1

Article End Page : 5


Introduction: Clinical biochemistry laboratory uses many techniques for measuring the concentration of specific biochemical substances. All these techniques are subject to interference from variety of sources. The aim of our study is to see the changes in concentration of serum bilirubin on exposure to special blue light of 440nm for six hours and to study the usefulness of photolysis for the elimination of bilirubin interference in the estimation of creatinine, glucose, and alkaline phosphatase.
Method: We have taken 218 samples and the samples were analyzed for the estimation of total bilirubin, direct bilirubin, creatinine, glucose and alkaline phosphatase before and after photolysis.
Results & Conclusion: There was significant decrease in the concentration of total and direct bilirubin after photolysis. There was a significant negative interference of creatinine and glucose and significant positive interference of alkaline phosphatase with an increasing concentration of bilirubin which was also eliminated by photolysis.

Interference, Photolysis, Analytes, Isomerization