International Journal of Clinical Biochemistry and Research

Use of deproteinizer to eliminate interference of high bilirubin on measurement of serum creatinine by the kinetic jaffe reaction

Full Text PDF Share on Facebook Share on Twitter

Author Details: Vijaysinh Parmar, Hiren Sanghani, Asha Khubchandani

Volume : 5

Issue : 2

Online ISSN : 2394-6377

Print ISSN : 2394-6369

Article First Page : 226

Article End Page : 229


Introduction: Serum creatinine is a useful diagnostic parameter of kidney function. Its concentration reflects glomerular filtration rate. Because of negative interference of bilirubin over creatinine level, may mislead the clinician. The most common and serious problems in the determination of serum creatinine concentration by the kinetic Jaffe method is the negative interference by bilirubin.
Materials and Methods: This cross sectional study was done on 160 patients which have high bilirubin level, taken from B. J. Medical College, Civil hospital, Ahmedabad from August 2013 to December 2013. These samples were studied for interference of total bilirubin on measurement of Serum creatinine level before and after treating with deproteinizer (trichloroacetic acid 0.55 mmol/ltr). Serum bilirubin and serum creatinine level were measured by modified Jendrassik & Groff’s method and kinetic Jaffe’s reaction respectively by using Erba XL-640 fully auto analyzer.
Results: In this study significant difference was found in Serum Creatinine level with the samples of high bilirubin content before and after treating with deproteinizer (trichloroacetic acid) (p<0>Preprecipitation of albumin along with bilirubin by trichloroacetic acid beforehand is suggested as an approach to correct the bilirubin interference.
Conclusion: From this study it is concluded that bilirubin have negative interference on serum creatinine level. And this is corrected by using deproteinizer trichloroacetic acid.

Keywords: Serum Creatinine, Serum bilirubin, Negative .intereference

Doi :-