International Journal of Clinical Biochemistry and Research


Iron chelating, reducing and anti-glycation activities of Coleus aromaticus leaves protein


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Article Type : Research Article

Author Details: Santhosh Kumar N,Kalaivanam K.N*,Aliya Nusrath,Dinesha Ramadas

Volume : 6

Issue : 2

Online ISSN : 2394-6377

Print ISSN : 2394-6369

Article First Page : 170

Article End Page : 175


Abstract

Introduction: Coleus aromaticus a medicinal plant is grown as weeds in the tropical countries like India. It has a wide range of active constituents, which has potent medicinal properties. The present study, the authors investigated the Iron chelating, reducing and anti-glycation properties of Coleus aromaticus leaves protein (CALP) in invitro model system. 
Materials and Methods: Proteins from the extract is isolated by Ammonium sulphate protein precipitation method. In vitro antioxidant studies were carried out by iron chelating activity and iron reducing power methods. The alpha amylase inhibitory activity, alpha glucosidase inhibitory activity and glucose uptake by yeast cells invitro studies was done. 
Results: The iron chelating activity and iron reducing power of the crude protein of Coleus aromaticus was compared with standard antioxidants like EDTA and Ascorbic acid. In anti-glycation studies, standard drugs Acarbose and Metformin was used. Results showed the percentage inhibition of ferrous ion chelating activity, increased with increase in concentrations of crude protein of Coleus aromaticus extract is comparable with standard antioxidants. Anti-glycation property and the inhibitory activity of CALP was compared with standard drugs. 
Conclusion: This study concluded that the Coleus aromaticus proteins possess significant anti-glycation activity and antioxidant activity when compared to standard antioxidants. Antioxidant activities increased with increasing concentrations in concentration dependent manner. It was found to be significant and valuable.

Keywords: Antioxidant, Coleus aromaticus leaves protein (CALP), Ferric ion reducing and ferrous ion chelating activity.

Doi :-https://doi.org/10.18231/j.ijcbr.2019.040