IP International Journal of Medical Microbiology and Tropical Diseases


Molecular characterization of vancomycin resistant enterococci from various clinical samples in a tertiary care hospital


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Article Type : Research Article

Author Details: V M. Somasunder*,Anitha C,S Senthamarai

Volume : 5

Issue : 2

Online ISSN : 2581-4761

Print ISSN : 2581-4753

Article First Page : 92

Article End Page : 94


Abstract

Aim: The aim of this present study was to investigate the molecular characterization of vancomycin resistant enterococci isolated from various clinical samples. Enterococci are aerobic and anaerobic gram positive cocci found in gastrointestinal tract of humans and other animals.
Materials and Methods: Enterococcal isolates were isolated from various clinical samples according to standard protocol and sample size of the study was 52. Vancomycin resistance genes VanA and VanB were detected using conventional PCR.
Results: The six isolates resistant to Vancomycin confirmed phenotypically were confirmed using Polymerase Chain Reaction.
Conclusion: in the present study conclude that, Vancomycin resistant enterococci is a major health problem in the coming years and hence it is necessary to take all adequate measures to identify the resistant strains.

Keywords: Vancomycin resistance, Enterococci and polymerase chain reaction.

Doi :-https://doi.org/10.18231/j.ijmmtd.2019.019