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Indian Journal of Microbiology Research


Bacterial Isolation and Their Antibiotic Susceptibility Pattern from Post-Operative Wound Infected Patients


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Author Details: Pooja Singh.Gangania. Varsha A. Singh, Sunil Sekhar Ghimire

Volume : 2

Issue : 4

Online ISSN : 2394-5478

Print ISSN : 2394-546X

Article First Page : 231

Article End Page : 235


Abstract

Background: Postoperative wound infections are found to be the cause of morbidity and mortality in the surgical patients. The wound that drain purulent discharge, with bacteria identified on culture is Postoperative infection. Wound draining pus, wounds that are opened by the surgeon; and wounds that the surgeon considers infected are considered postoperative wound infection.
Objectives: Bacterial isolation and antibiotic susceptibility patternof postoperative wound infected patients and to find the correlation between their age, sex and the site of infection.
Material and methods: 200post surgical patients with wound infections admitted in different surgical wards wereincluded. Pus and serous fluid samples from the wounds were collected. Gram staining &culture was done via conventional techniques followed by AST by Kirby Bauer disk diffusion technique.
Results:Gram negative (27.5%) were predominant over gram positive (11.5%).In gram positive organism maximum isolates were S.Aureus followed by Enterococcus and streptococcus In gram negative organism’s maximum isolate was E.coli followed by Pseudomonas Aeruginosa ,klebsiella& Proteus.
Conclusion:E.coli (14.5%) was the commonest pathogen isolated due to more laprotomy surgical procedures followed by equal rate of Staphyalococcusaureus (7%) and Pseudomonas aeruginosa (7%), Klebsiella (3.5%), Proteus (2.5%), Enterococcus (2.5%) and Streptococcus (2%). First generation of Cephalosporins and most of the Aminoglycosides were sensitive against gram positives whereas successive generations of cephalosporins were sensitive against gram negatives including some penicillin drugs like Ampicillin, Amoxicillin, and Pipracillin-Tazobactum which were sensitive for both gram positive and gram negative organisms.

Key words: Postoperative wound infection, surgical site infection, Bacterial isolation, Bacterial characterization, Antibiotic susceptibility testing (AST)