Indian Journal of Microbiology Research

Resident microbial flora amongst nursing staff as a source of nosocomial infection

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Author Details: Veena Maheshwari, NavinChandra M. Kaore, Vijay Kumar Ramnani, Rajesh Shah

Volume : 3

Issue : 3

Online ISSN : 2394-5478

Print ISSN : 2394-546X

Article First Page : 329

Article End Page : 332


Background: Infections due to hospital-acquired microbes is an evolving problem worldwide. Prevalence as high as 19%, of these infections in developing countries poses a challenge to health care providers. A major source of horizontal transmission of the nosocomial infections are thought to be bacterial contamination of palm of hand of health care providers like doctors and nurses. Hand hygiene is the single most cost effective preventive measure against hospital acquired infections and education is an important tool to ensure its implementation. The present study was undertaken, to demonstrate the presence of resident microbial flora on the hands of nursing staff after duty hours working at various critical areas in a tertiary care hospital with their antibiogram.
Materials and Methods: This cross sectional analytical study was carried out after ethical clearance from June to August 2013on 100 nursing staff of either sex working in critical areas of tertiary care hospital after due informed consent from the nurses. A sterile swab moistened with sterile normal saline was used to collect the swabs from dominant(working) hands after working hours. The collected swabs were processed for isolation, identification and antibiotic sensitivity of organisms.
Results: Out of 100 swabs collected from the dominant hands of nurses working in critical areas, 83 showed the growth. All the samples cultured shows growth of more than 2 organisms. The major Gram Positive pathogenic organisms grown were Coagulase negative Staphylococcus spp. 40 (48.19%) followed by Staphylococcus aureus 30 (36.14%) whereas amongst Gram Negative Klebsiella spp. 5(6.025%) and Pseudomonas aeruginosa 4(4.81%) predominated..
Conclusions: In the present study, the level of contamination of nursing staff was found to be high. Resident flora consisting of multi drug resistant pathogenic organisms can be greatly reduced by an increase in hand-hygiene awareness coupled with organizational interventions. We recommend to closely monitor hand hygiene practices for effective intervention strategies.

Dominant hands, Nursing staff, HAI, Resident microbial flora, Multidrug resistant organisms