Indian Journal of Microbiology Research

Characterization and antimicrobial susceptibility pattern of non-fermenting gram negative bacilli from various clinical samples in a tertiary care hospital

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Author Details: Gomathi Maniyan, Dillirani Vedachalam, Nithya Chinnusamy

Volume : 3

Issue : 4

Online ISSN : 2394-5478

Print ISSN : 2394-546X

Article First Page : 387

Article End Page : 391


Background: Non fermenting Gram Negative Bacilli (NFGNB) once considered as contaminants have now emerged as a major cause of life threatening nosocomial infections and as multidrug resistant pathogens. Aim: To isolate and identify the NFGNB and their antimicrobial susceptibility pattern and to detect the Extended Spectrum Betalactamases (ESBL) among the isolated non-fermenters.
Materials and Methods: This Cross sectional study conducted in Medical College and Hospital for one year and samples collected like pus, urine, endotracheal aspirates, blood, sputum and body fluids were identified using standard protocol, which includes Grams staining, test for motility, catalase test, oxidase test, OF test and various biochemical reactions.
Results: Out of 110 clinically significant isolates of non-fermenters, 54(49%) were Pseudomonas aeruginosa, 36(32.7%) Acinetobacter baumannii, 8(7.3%) Acinetobacter lwoffi, 6(5.4%) S.maltophilia and Pseudomonas stutzeri and Burkholderia cepecia 3(2.8%). The antimicrobial susceptibility pattern revealed maximum resistance to Gentamycin (61.8%), Cotrimoxazole (60%), followed by Ciprofloxacin(50.9%) and Cefotaxime(47.3%). Sensitivity to Polymyxin B (100%) followed by Imipenem and Meropenem (75.5%). ESBL production was 18.18%.
Conclusion: Pseudomonas aeruginosa and Acinetobacter baumannii were the most common NFGNB isolated in this study. Difference in antimicrobial susceptibility by nonfermenters pose a great problem in treating these infections. ESBL production by these organisms lead to high morbidity and mortality.

Non-fermenter, Antimicrobial susceptibility, ESBL, Pseudomonas, Acinetobacter