Indian Journal of Microbiology Research


Molecular characterization of biofilm producing Staphylococci isolated from Central Venous Catheters in a tertiary care hospital


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Author Details: Vasanthi Rompicherla, Priyadarshini Shanmugam, Shameem Banu Abdul Sattar

Volume : 3

Issue : 4

Online ISSN : 2394-5478

Print ISSN : 2394-546X

Article First Page : 432

Article End Page : 435


Abstract

Introduction: Medical device associated infections are a major cause of substantial morbidity, due to wide usage of intravenous catheters in various medical disciplines like renal dialysis units and in cardiology department. Several species of Staphylococcus which usually occur as normal microbial flora on mucosa and skin are a frequent cause of device associated infections due to biofilm production. This polysaccharide slime synthesis is mediated by ica operon.
Aims and Objectives:  To determine the prevalence of biofilm producing strains of Staphylococci isolated from Central Venous cannulas by phenotypic and genotypic methods.
Materials and Methods: 220 Central Venous devices were received and processed, of which 30 yielded growth. Staphylococci were identified and biofilm production was determined by the tissue culture plate method and by PCR. Genotyping of biofilm producing Staphylococcal species was done for detection of ica genes (intercellular adhesion) by conventional PCR.
Results: A total of 220 central venous catheter tips were collected, out of which 30 bacterial organisms were isolated. Among these isolates, 20 (67%) were Staphylococcus species which were considered for phenotyping by tissue culture method and genotyping by conventional PCR. Out of these, 10 (50 %) Staphylococcal species were phenotypically positive for biofilm production and 11 (55%) showed the presence of ica gene by PCR