Indian Journal of Microbiology Research


Incidence of Methicillin Resistant Staphylococcus aureus and its antibiotic susceptibility pattern in a tertiary care hospital


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Author Details: Sanjay Mehta

Volume : 4

Issue : 1

Online ISSN : 2394-5478

Print ISSN : 2394-546X

Article First Page : 40

Article End Page : 43


Abstract

A total of 250 Staphylococcus aureus strains were isolated from various clinical samples over a period of 9 months at M. P. Shah Medical College, Jamnagar to identify the incidence rate and antibiogram of Methicillin Resistance Staphylococcus aureus (MRSA). All strains of S. aureus were tested for susceptibility to methicillin / oxacillin by disk diffusion method and minimum inhibitory concentration (MIC) of methicillin was determined by agar dilution method as per standard procedures. Antibiogram study of MRSA strains was performed. Out of total 250 S. aureus strains, 145 (58%) strains were methicillin and oxacillin resistant, having methicillin MICs of ≥ 16 µg / ml, 100 (40%) strains were methicillin and oxacillin susceptible having methicillin MICs of ≤ 4 µg /ml. 5 (2%) strains were borderline susceptible having methicillin MICs of 8 µg / ml. Borderline susceptible strains were susceptible to co-amoxiclave and were not considered as methicillin resistant strains. All 145 (100%) MRSA strains were resistant to penicillin G, 139 (96%) were resistant to erythromycin, 136 (94%) were resistant to gentamicin, 80 (55%) were resistant to amikacin, and 35 (24%) were resistant to rifampicin. All MRSA strains were susceptible to vancomycin.

Keywords:
Staphylococcus aureus, MRSA, MIC, Antibiogram, Antibiotic Resistance