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Indian Journal of Microbiology Research

Ethanol production from sugarcane bagasse hydrolysate using newly adapted strains of Meyerozyma guilliermondi CBA-524 and Pichia kudriavzevii CBA-519

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Author Details: Marcia Jaqueline Mendonca Maciel, Karol de Souza Barbosa, Luciana Araujo Cassa-Barbosa, Spartaco Ast

Volume : 4

Issue : 2

Online ISSN : 2394-5478

Print ISSN : 2394-546X

Article First Page : 182

Article End Page : 186


New strains of the yeasts Meyerozyma guilliermondi CBA-524 and Pichia kudriavzevii CBA-519, capable of growing on sugarcane bagasse hydrolysate, have a great potential for second-generation bioethanol processes. Meyerozyma guilliermondi CBA-524, achieved a maximum ethanol concentration of 3.01g/L corresponding to a yield of 0.26 g ethanol per g of sugar and a productivity of 0.125 g/l/h, after 24 h of cultivation. Pichia kudriavzevii CBA-519 exhibited a prolonged fermentation time on bagasse hydrolysate resulting in an ethanol yield of 0.26 g/g and a productivity of 0.015 g/l/h after 192 h of fermentation. The ethanol yield and the volumetric productivity using the adapted P. stipitis CBS6054 standard were 0.34 g/g and 0.083 g/l/h, respectively, after 48 h of cultivation. Furthermore, the newly adapted M. guilliermondi CBA-524 demonstrated significantly enhanced tolerance to sugarcane bagasse hydrolysate, exhibiting a maximum in ethanol concentration after 24 h of fermentation. More adapted yeasts and improved fermentation conditions for this yeast can be of great importance for the increased production of ethanol using sugarcane bagasse hydrolysate.

Hydrolysate, Bioethanol, Meyerozyma guilliermondi, Pichia kudriavzevii.