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Indian Journal of Microbiology Research

Study of diagnostic efficacy of widal slide agglutination test against widal tube agglutination test in enteric fever

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Author Details: Roshni Pranav Patel, Minalben Bharatkumar Trivedi

Volume : 4

Issue : 3

Online ISSN : 2394-5478

Print ISSN : 2394-546X

Article First Page : 267

Article End Page : 269


Introduction: Enteric fever is a major and persistent public health problem in India. The slide widal is most commonly performed rapid method to diagnose due to its convenience, but its results are required to be compare with more standardly established tube widal test for its actual diagnostic significance. This study aims to compare the result of slide widal test with tube widal test to ascertain any significant difference between two methods.
Materials and Method: The 100 pre-collected serum samples with widal test request were received for the study over the six months period. Comparison of slide widal agglutination test with tube widal agglutination was done considering tube widal as a standard.
Result: The slide widal agglutination test shows false positive result in 5.3% and 48.1% serum samples with S.typhi O antigen & S.typhi H antigen, respectively. The Positive Predictive Value of widal slide agglutination test for O antigen and H antigen is 94% and 52% respectively.
Conclusion: The slide widal agglutination test showed high false positive rate and low positive predictive value especially with S.typhi H antigen. Therefore, the result of slide widal agglutination needs to be confirmed by tube widal agglutination test.

Agglutination tests, Comparison, Slide, Tube, Widal test