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Year 2019

Volume: 6 , Issue: 3

Print ISSN:-2394-546X

Online ISSN:-2394-5478

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Indian Journal of Microbiology Research


Evaluation of antimicrobial resistance in urinary isolates of member of enterobacteriaceae among woman attending Tertiary Care Hospital


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Author Details: Vinod Rai, Lal Pranay Singh, Vijay K. Ramnani

Volume : 4

Issue : 3

Online ISSN : 2394-5478

Print ISSN : 2394-546X

Article First Page : 274

Article End Page : 278


Abstract

Introduction: 1. Urinary Tract Infections (UTIs) are one of the most common urogenital infections and affects all age groups including men, women and children worldwide.
2. Urinary tract infections are a major public health problem in terms of morbidity and financial cost and incur the highest total health care cost among urological diseases.
3. UTI represents one of the most common disease/syndrome encountered in medical practice today with an estimated 150 million UTIs per annum worldwide.
Materials and Method: A total of 300 mid-stream urine specimen from woman patient received in the department after collection in a sterile container were received processed by semi quantitative culture technique using a standard calibrated loop (diameter 0.04mm) on blood agar (BA) and MacConkey agar (MA). After 24hr of aerobic incubation at 37°C, culture growth showing significant bacteriuria were included in the study for further processing. Standard biochemical test of identification were used to identify bacterial isolates. Antimicrobial susceptibility was determined by Kirby-Bauer disk diffusion method as per CLSI Recommendations.
Result: Out of 300 urine samples received Significant Growth was seen in 134 (44.67%) samples, whereas 166 (55.33%) samples showed either Insignificant Growth (polymicrobial) or showed no Growth.
Conclusion: The restricted use of antibiotics can lead to the withdrawal of selective pressure and the resistant bacteria will no longer have a survival advantage against these antibiotics. Hence, there is a need to formulate strategies to detect and prevent the emergence of resistance for an effective treatment of the urinary tract infection.

Keywords:
Urinary Tract Infections, Member of enterobacteriaceae, E. coil, Klebsiella spp., Staphylococcus spp., Enterobacter spp