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Indian Journal of Microbiology Research

Bacteriological profile of post surgical wound infections: A one year retrospective study from a government orthopaedic hospital

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Author Details: Pratibha Shamanna, Praveen Kumar, Vanishree

Volume : 4

Issue : 3

Online ISSN : 2394-5478

Print ISSN : 2394-546X

Article First Page : 291

Article End Page : 294


Post-operative wound infection is a severe problem in the surgical specialization and is an important cause of morbidity and mortality. In most of these wound infections the causative agents arise from the endogenous flora of the patient’s skin, mucous membranes or hollow viscera. Advances in the control of infection have not completely eradicated this problem because of development of drug resistance.
Aim: The main objective of this study was to determine the causative aerobic bacteria and their antimicrobial susceptibility from pus specimens of post-operative wound infections.
Materials and Method: The study was conducted using pus culture and sensitivity reports collected retrospectively from the records maintained in the Department of Microbiology over a period of one year from January 2015 – January 2016 in our Hospital. A total of 290 pus samples were received and were processed by doing Gram stain, Culture, Biochemical identification and Susceptibility testing. Out of 290 samples 218 samples were from IPD and 72 were from OPD.
Results: During the study period, 290 pus culture and sensitivity reports were analyzed (99 samples (34.14%) of the samples showed no growth). 191 samples (65.86%) showed growth i.e. culture positive. Pseudomonas aeruginosa was most frequently isolated organism (21.63%) followed by Staphylococcus aureus (14.65%). Pseudomonas species were mostly sensitive to Imipenem (70%) & Meropenem (50%). Staphylococcus aureus were mostly sensitive to Netilmicin (100%), Linezolid (100%) followed by Chloramphenicol (97%), Tetracycline (94%) & Clindamycin (91%). Other gram negative isolates were Enterobacter species, Klebsiella species and Escherichia coli.
Conclusion: Knowledge about the bacteriological profile and their antibiotic susceptibility pattern of post-operative wound infections can serve as a useful tool for the clinicians to start empirical treatment of patients at the earliest, according to local pattern and emerging multidrug resistance and also to give importance to strict infection control practices and periodic surveillance.

Bacteriology, Pus culture, Post-surgical wound infection, Sensitivity