Indian Journal of Microbiology Research


A study of detection of Neisseria gonorrhoeae among HIV positive and HIV negative patients by conventional and molecular methods


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Author Details: Nawaz Umar, Bilal Ahmad, S.B. Siddish

Volume : 4

Issue : 4

Online ISSN : 2394-5478

Print ISSN : 2394-546X

Article First Page : 367

Article End Page : 372


Abstract

Introduction: Gonorrhea infection is caused by a Sexually Transmitted Infection (STI) that infects both men and women. Based on the clinical manifestations it is difficult to diagnosis Neisseria gonorrhoeae. Conventional diagnosis techniques like gram stain and culture for the diagnosis of gonorrhea are inexpensive but have poor sensitivity especially in asymptomatic patients. Rapid diagnosis is important for the control of the disease, PCR method is one such rapid method.
Materials and Method: A total of 611 specimens were collected from different anatomical sites in men and women of reproductive age group who attend the STD and non-STD clinic at Khaja Bandanawaz Institute of Medical Sciences, Gulbarga, Karnataka, during the period of June 2013 to June 2016. Direct smears for gram staining, Culture, Biochemical tests and molecular method (PCR) were performed.
Conclusion: in the present study, the comparison between conventional and Molecular methods was done; gram staining was less sensitive than culture and PCR. Culture on chocolate agar [CA] was not showing 100% results compare to the Modified Thayer-Martin Media [MTM] on which all 55 specimens were grown, but PCR showed 94.5% result even though there was less specimen in the swabs. PCR technique for the detection of orf1 gene of Neisseria gonorrhoeae directly from clinical samples is a promising investigative method. However, culture on selective media (MTM) is the method of choice for the identification of Neisseria gonorrhoeae and susceptibility testing, particularly in developing countries like India.

Keywords:
ONPG, PCR, MTM, Neisseria gonorrhoeae, Sexually Transmitted Infection