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Indian Journal of Microbiology Research

Comparison of Rapid Slide Culture with Culture in L-J Medium and MGIT for Isolating Mycobacterium tuberculosis from Bronchoalveolar Lavage Fluid

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Author Details: Shweta Sharma, Biswaroop Chatterjee, Anurag Bhargava, Aarti Kotwal, Girish Sindhwani

Volume : 4

Issue : 4

Online ISSN : 2394-5478

Print ISSN : 2394-546X

Article First Page : 384

Article End Page : 387


Aims: Rapid Slide Culture (RSC) was compared with the standard diagnostic strategy of simultaneous culture in Löwenstein-Jensen medium (L-J) and Mycobacterial Growth Indicator Tubes (MGIT™) with regard to diagnostic accuracy, turnaround time and cost, for isolating M. tuberculosis (MTB) from bronchoalveolar lavage (BAL) specimens.
Materials and Method: Centrifuged deposits of 75 consecutive smear-positive and 75 randomly selected smear-negative BAL specimens were subjected to RSC as well as culture in both L-J and MGIT. Isolates were identified as MTB by standard methods.
Results: One hundred and ten (73.3%) specimens were culture-positive by L-J and / or MGIT; 99 (90%) of these were RSC-positive too. No RSC-positive specimen was culture-negative on both L-J and MGIT. Thus, RSC demonstrated an overall sensitivity, specificity, PPV and NPV of 90%, 100%, 100% and 78% respectively. Significantly, 25 (33%) smear-negative specimens were RSC-positive, indicating the ability of RSC to isolate MTB from specimens with low bacillary loads. The mean time to positivity was seven, 35 and 24 days for RSC, L-J, and MGIT respectively. The cost per test was the equivalent of USD 1.82, 1.34, and 10.42 for RSC, L-J and MGIT respectively.
Conclusion: Rapid Slide Culture proved to be a rapid, effective and low-cost method for culturing MTB from bronchoalveolar lavage fluid, when compared with conventional culture in L-J medium and MGIT.

Tuberculosis; Bronchoalveolar lavage; Diagnosis; Culture