Indian Journal of Microbiology Research


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Author Details: Sarika Kombade, Gopal N. Agrawal

Volume : 2

Issue : 2

Online ISSN : 2394-5478

Print ISSN : 2394-546X

Article First Page : 120

Article End Page : 125


Background and objectives: Nonfermenting gram-negative bacilli (NFGNB) are ubiquitous in the nature. They are considered as environmental contaminants and most of them have emerged as important nosocomial pathogens. So this study was undertaken to identify the various NFGNB with its antimicrobial susceptibility pattern of specimens from patients admitted to Intensive care unit (ICU) in our hospital

Methods: Total 107 NFGNB were identified during the study period of September 2009-October 2011. All isolates were identified using a standard conventional method like motility, oxidase production; oxidation-fermentation test etc. and failing to acidify butt in triple sugar iron medium. Antibiotic susceptibility testing was performed with the help of the Kirby-Bauer disc diffusion method and MBL detection done by DDST and Modified Hodge test.
Result: P. aeruginosa (16%) was the commonest isolate obtained followed by A. baumannii (13.5%). NFGNB were maximum susceptible to imipenem (80.4%), piperacillin tazobactum (71.9%) and amikacin(52.3%). P.aeruginosa and A. baumannii were 90% and 60.6% sensitive imipenem and 85% and 54.5% to piperacilli-tazobactum. 19.6% MBL producers were obtained in all nonfermenters. Maximum MBL production was obtained in Acinetobacter spp. (39.4%) followed by S. maltophilia (20%) and P. aeruginosa (10.0%)
Interpretation & conclusion: Multidrug resistance infection in ICU is emerging; we also obtained 19.6% MBL producers in all NFGNB. Local antibiotic policy should be generated to prevent the emerging MDR in NFGNB.

Keywords: Nonfermenters, Intensive Care Unit, MDR, Antibiotic sensitivity test