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Indian Journal of Microbiology Research


Screening for group B streptococci in antenatal women: Isolation and its antibiogram


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Author Details: Balan K., Abirami Lakshmy, Ashwini MA, Vijayalakshmi TS., Sheila Doris Devamani

Volume : 5

Issue : 2

Online ISSN : 2394-5478

Print ISSN : 2394-546X

Article First Page : 151

Article End Page : 153


Abstract

Group B streptococci(GBS) is one of those pathogens causing neonatal septicaemia and neonatal meningitis. Objective of this study is to screen antenatal women for Group B Streptococcus colonization and its antibiotic sensitivity pattern by Kirby bauer disk diffusion method. This study was carried out in Department of Microbiology during a period of 4 months.A total of 100 pregnant women attending antenatal clinics in the age group 20-40 years were included in the study. Two vaginal swab were taken aseptically and immediately transported to the laboratory for processing. One swab for direct gram staining and other swab was inoculated into sheep blood agar and incubated at 37 ºC for 24-48 hours. Identification of organism was based on gram staining, colony morphology, catalase reaction, Christie Atkins Munch Peterson(CAMP) and Hippurate hydrolysis test. Results of this study show that prevalence rate for GBS colonization is 8%.Pregnant women less than 20 years of age and primigravida are more commonly colonized with Group B Streptococci. All the isolates were sensitive to Penicillin, Erythromycin, Ampicillin, Gentamicin, Ceftriaxone and Chloramphenicol. Hence all pregnant women should be screened for Group B Streptococci carriage between 35 and 37 weeks of gestation and intrapartum antibiotic prophylaxis be given to colonized women at the time of labour or rupture of membranes.

 

Keywords: Group B Streptococci, Antenatal pregnant women, Prevalence, Antibiotic susceptibility pattern

Doi :-https://doi.org/10.18231/2394-5478.2018.0031