Indian Journal of Microbiology Research


Clinical and microbiological profile of infective keratitis and their antibiotic sensitivity


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Author Details: Ashwini S Waghmare,Praveen Kumar Sadanand*

Volume : 6

Issue : 1

Online ISSN : 2394-5478

Print ISSN : 2394-546X

Article First Page : 11

Article End Page : 14


Abstract

Introduction: Microbial keratitis is a common, potentially vision-threatening ocular infection that may be caused by bacteria, fungi, or parasites. Successful treatment of corneal ulcer requires proper diagnosis and early institution of intensive therapy with appropriate antimicrobial agents.
Aim: To determine specific pathogenic organism and clinical risk factors for infective Keratitis.
Materials and Methods: Patient with clinical features of infective keratitis attending Ophthalmology OPD were included whose corneal scrapings were subjected to Gram staining, KOH Mount and culture sensitivity. Statistical analysis done by using appropriate statistical methods.
Results: 40 cases were evaluated. 23 were males and 17 females. Age ranged from 21 to 70 years. History of trauma was present in 47.5% of patients, ocular surface disease was present in 10% while diabetes was present in 10% of patients. Based on culture reports, bacterial and fungal keratitis found in (65%) 26 patients. There was no organism isolated from rest of the 14 samples (sterile keratitis). Bacteria were isolated in 14 samples and in 12 samples fungal growth was isolated. The commonest bacterial isolate were Staphylococcus aureus followed by Pseudomonas, Coagulase negative staphylococcus and Klebsiella. Aspergillus species was isolated in 7 patients and fusarium species in 4 patients. Gram positive isolates were maximally sensitive to Ofloxacin, gram negative to Gentamicin.
Conclusion: Staphylococcus aureus was the most common bacterium while Aspergillus was the most common fungus isolated. Trauma was the commonest risk factor for corneal ulcer.

Keywords: Corneal ulcer, Staphylococcus, Aspergillus, Trauma, Culture.

Doi :-https://doi.org/10.18231/2394-5478.2019.0003