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Indian Journal of Microbiology Research

Expression of Assimilatory Nitrate and Nitrite Reductase of Rhizobium meliloti

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Author Details: Anup Kumar Halder, P. K. Chakrabartty

Volume : 2

Issue : 3

Online ISSN : 2394-5478

Print ISSN : 2394-546X

Article First Page : 133

Article End Page : 137


Purpose: To investigate the expression of assimilatory nitrate- and nitrite reductase activity of Rhizobium meliloti SU 47.

Methods: Rhizobium cell were grown in medium, after 20 hrs cells were washes with potassium phosphate buffer and assayed the enzyme activity through nitrite estimation.
Result: Expression of assimilatory nitrate- and nitrite reductase was observed in GTS/glutamate medium. The enzymes were inducible in nature since it expressed only in presence of nitrate or nitrite. Ammonium inhibited the enzyme activity. Cyanate acted as a gratituous inducer of both the enzymes. Molybdenum acts as modulator whereas tungstate as an inhibitor of the enzyme.
Conclusion: Both the enzymes, assimilatory nitrate- and nitrite reductases expressed in GTS/glutamate medium in presence of nitrate.
Key Words: Assimilatory nitrate and nitrite reductase, Rhizobium meliloti, Cyanate