Indian Journal of Microbiology Research

Analysis of urine culture isolates from microbiology laboratory of a tertiary care Hospital

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Article Type : Research Article

Author Details: Rahul B Lunagaria,Nisarg A Trivedi*

Volume : 6

Issue : 2

Online ISSN : 2394-5478

Print ISSN : 2394-546X

Article First Page : 106

Article End Page : 108


The study of urine culture isolates was conducted on samples received to microbiology laboratory of GMERS Medical College attached General Hospital, Gandhinagar during the period of December 2015 to November 2016.
Only isolates from urine samples of non catheterized patients having colony count of >105 CFU/mL were included for analysis. Analysis of the species distribution and antibiotic sensitivity pattern was done with the help of WHONET 5.6 software.
Out of 160 isolates identified from 801 urine samples (positivity rate of 19.98%), most common were coliforms (71.88%) followed by pseudomonas spp. (8.75%), candida spp. (8.75%) etc. Female patients (22.81%) had higher rate of urinary tract infection as compared to males (17.45%). Enterobacteriaceae group was most resistant among all isolates. Amikacin (85%) and imipenem (82%) were most sensitive antibiotics in Enterobacteriaceae group while polymyxin-B (100%) and aztreonam (93%) were most sensitive in pseudomonas spp. Antimicrobial susceptibility testing and analysis was done for gram positive cocci also.
Enterobacteriaceae are the leading cause of urinary tract infection worldwide and also most resistant among all isolates. Cautious usage of antimicrobial agents is required to reduce the burden of increasing drug resistance in UTI.

Keywords: Urinary tract infection, Antimicrobial sensitivity, Coliform, Gender difference.

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