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IP International Journal of Ocular Oncology and Oculoplasty

Role of MDM2 SNP309 gene mutation in pterygium: a pilot study

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Author Details: Heena Sareen, Pawan Prasher, Sujan Singh, Ashwani Kumar Thukral, Anup Kumar Kesavan

Volume : 3

Issue : 2

Online ISSN : 2581-5016

Print ISSN : 2581-5024

Article First Page : 108

Article End Page : 111


Introduction: Pterygium is a common, vision impairing ocular surface disease whose etiopathogenesis is not yet fully established. Several factors, including ultraviolet radiation, heat, dry climate, microtrauma due to dust particles, and viral infections have been attributed to play a role in its pathogenesis. Recent studies have suggested that ptergyium might have a neoplastic origin. MDM2 SNP309 mutation has been associated with various tumors, however, its role in the pathogenesis of pterygium is not known.
Objectives: To study the association between MDM2 SNP309 mutation and pterygium.
Materials and Methods: In the present study, 37 pterygium patients and 49 controls were screened for MDM2 SNP309 mutation. Peripheral venous blood samples were collected from the study participants and the genomic DNA was isolated. This genomic DNA was used for polymerase chain reaction (PCR) amplification of MDM2 SNP309 mutation using the following set of primers: forward 5’-CGGGAGTTCAGGGTAAAGG-3’ and reverse 5’-TCGGAACGTGTCTGAACTTG-3’.
Results: In the pterygium group, the frequency of genotypes GG, GT and TT were 32.6%, 40.8% and 26.5%; whereas in the controls it was 27.0%, 35.1% and 37.8% respectively. There was no statistically significant difference between the two groups in the genotype frequency of MDM2 SNP309 polymorphism (p>0.05).
Conclusions: The results showed no significant difference between the ptergyium patients and controls in the genotype frequency of MDM2 SNP309 polymorphism.

Keywords: MDM2 SNP309  polymorphism, Polymerase chain reaction, Pterygium