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International Journal of Pharmaceutical Chemistry and Analysis

Analytical Method Development and Validation for Quantitative Estimation of Torsemide in Bulk and Pharmaceutical Dosage Form by RP-HPLC

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Author Details: Amol. Y. Ghodke, Bhagwat. N. Poul, Mohini. B. Sorde

Volume : 1

Issue : 1

Online ISSN : 2394-2797

Print ISSN : 2394-2789

Article First Page : 6

Article End Page : 13



A simple, specific, precise and accurate Reverse Phase High Performance Liquid Chromatography (RP-HPLC) method was developed and validated for the quantitative estimation of Torsemide in Bulk and Pharmaceutical dosage form. The proposed RP-HPLC method was carried out on Zorbax C18 (250x4.6mm), 5μm column with mobile phase phosphate buffer and methanol (50:50) (v/v). The pH of phosphate buffer was adjusted by Ortho- phosphoric acid to 3.5. The flow rate was 1.3 mL/min and the detection wavelength was 288nm. The retention time of torsemide was found at 6.0±0.2min. The method was validated for specificity, precision, accuracy, linearity and robustness. The linearity range was 10-30 μg/mL and correlation coefficient (r2) was found to be 0.9980. The mean % recovery for Torsemide was found to be 99.80. The developed method could be employed for the routine analysis of Torsemide from different formulations and for the Torsemide calculations as well.