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International Journal of Pharmaceutical Chemistry and Analysis

Simultaneous Determination of Dexibuprofen and Tramadol HCl by HPTLC Method

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Author Details: Prachi Pimple

Volume : 4

Issue : 3

Online ISSN : 2394-2797

Print ISSN : 2394-2789

Article First Page : 79

Article End Page : 82


Introduction: Aim of the study is to develop simple, specific, accurate HPTLC method for simultaneous determination of the Dexibuprofen and Tramadol HCl in pharmaceutical dosage form.
Materials and Method: Stationary phase was precoated silica gel 60 F254. The Mobile Phase used was mixture of Chloroform: toluene: ethanol: glacial acetic acid (8:4:1:0.5 v/v/v/v). The detection of the spots was carried out at 264nm and 271nm for Dexibuprofen and Tramadol HCl respectively.
Result: The method was validated in terms of linearity, accuracy, precision and specificity. The Limit of detection for Dexibuprofen and Tramadol HCl was found to be 1µg and 0. 2 µg respectively. The Limit of quantification for Dexibuprofen and Tramadol HCl was found to be 1.6µg and 0.4µg respectively.
Conclusion: The method is free from the interferences due to excipients present in the formulation and can be successfully used for determine drug in pharmaceutical dosage form.

Dexibuprofen, Tramadol HCl, HPTLC method, Simultaneous determination