Indian Journal of Pathology and Oncology


Spectrum of histopathological diagnosis of lymph node biopsies and utility of immunohistochemistry in diagnosis of lymphoma: A 5 year retrospective study from a tertiary care Centre in South India


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Article Type : Research Article

Author Details: Shanmuga Priya S,Rajalakshmi V*

Volume : 6

Issue : 3

Online ISSN : 2394-6792

Print ISSN : 2394-6784

Article First Page : 434

Article End Page : 439


Abstract

Introduction: The lymph node biopsy plays an important role in establishing the cause of lymphadenopathy. The distribution of lymphomas is varied in different geographic regions. The 2016 revision World Health Organization classification of lymphoid neoplasms is used to classify lymphomas.
Aims: To study retrospectively the spectrum of histopathological diagnosis in lymph nodes at our institution, diagnostic utility of immunohistochemical markers in the subtyping of both nodal and extranodal lymphomas and to correlate the histopathology and immunohistochemistry.
Materials and Methods: We retrospectively reviewed lymph nodes between 2012 and 2017, analysed the distribution of cases and classified lymphoma according to the revised 2016 World Health Organization classification of lymphoid neoplasms and compared with other studies.
Results: The distribution of various cases from 235 lymph nodes were as follows: 27% of granulomatous lymphadenitis, 25% of reactive lymphoid hyperplasia, 24% of metastatic deposit, 17% of lymphoma, and 7% of miscellaneous category that included progressive transformation of germinal centres, Kimuras disease, extramedullary hematopoiesis, kikuchi lymphadenitis, neuroendocrine tumor, schwannoma. B-cell lymphomas outnumbered T- cell lymphomas. The statistical parameters were compared between histopathology and immunohistochemistry. Sensitivity, specificity, diagnostic accuracy, positive predictive value and negative predictive value were calculated.
Conclusions: Lymph node biopsy helps in establishing the cause of lymph node enlargement. The distribution of lymphomas in India is varied when compared to the rest of the world. Immunohistochemistry plays an important role in subtyping lymphomas.

Keywords: Lymphoma, WHO classification, Immunohistochemistry, Lymph node.

Doi :-https://doi.org/10.18231/j.ijpo.2019.083