Indian Journal of Pharmacy and Pharmacology


Prevalence, antibiotic resistance pattern and identification of extended spectrum beta-lactamase producing Klebsiella pneumoniae by phenotypic and genotypic method from various clinical samples


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Author Details: Shireen Rana,Bilal Ahmad Mir*,S B. Siddesh

Volume : 5

Issue : 4

Online ISSN : 2393-9087

Print ISSN : 2393-9079

Article First Page : 202

Article End Page : 206


Abstract

The present study is about ESBL producing Klebsiella pneumoniae which is diagnosed by phenotypic and genotypic methods. A total of 5055 properly collected, well labelled samples, received in the Microbiology laboratory were processed following standard protocol. Total 5055 samples, 805 (16.0%) Klebsiella pneumoniae were isolated. 226/3415 (6.64%) urine, 218/649 (33.6%) wound, 127/342 (37.1%) blood, 215/604 (35.6%) pus and 18/45(40.0%) sputum samples tested positive for Klebsiella pneumoniae. The occurrence of ESBL producers among Klebsiella pneumoniae in the current study is 293/805 (36.4%). Highly resistant drugs for Klebsiella pneumoniae ESBL isolates were Ampicillin (100%), Piperacillin (100%), Amoxycillin + clavulanic acid (66.2%). Non ESBL producing isolates were less resistant to the same antibiotics. Out of 805 isolated 285 (35.4%) and 293 (36.3%) were identified as ESBL producers by double disc synergy test and phenotypic confirmatory test respectively. Samples identified as ESBL producers were confirmed by E test. Detection of gene among these isolates, single bla gene could be detected in 59 (20.1%), two genes in 71 (24.2%) and three genes in 156 (53.2%) isolates. blaCTX-M-15 was the only gene found in 8 (4%) of the Klebsiella pneumoniae isolates tested, whereas blaSHV alone was found in 42 (14.3%) isolates. Among the combination of two bla genes, the combination of blaSHV and blaCTX-M-15 was found in 54 (18.4%) isolates. This study establishes that considerable genetic diversity and variations in the distribution of ESBL producers and their bla genes exist in the clinical isolates of K. pneumoniae across Karnataka.

Keywords: ESBL, Klebsiella pneumoniae, CLSI guidelines, Gene.

Doi :-https://doi.org/10.18231/2393-9087.2018.0042