IP Archives of Cytology and Histopathology Research


Micronucleus and its significance in spectrum of cervical lesions


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Author Details: Kalpana Kumari MK, Kavya J

Volume : 2

Issue : 1

Online ISSN : 2456-9267

Print ISSN : 2581-5725

Article First Page : 5

Article End Page : 9


Abstract

Introduction: Cervical cancer remains second most common cancer among the women worldwide despite effective screening methods. Micronuclei (MN) test on exfoliated cells have been one among tests used in cancer screening. They are intracytoplasmic inclusions derived from chromatin fragments reflecting chromosomal instability and is noted in high frequency in carcinogen-exposed tissues long before any clinical symptoms are evident.
Objective: To evaluate the role of micronuclei in inflammatory, preneoplastic and neoplastic lesions of cervix.
Methodology: A prospective study was done on 80 cervical smears received in the department of pathology, from March 2016 to June of 2016, which were stained with papanicolau stain. Of which 75 smears were included and 5 were excluded. The smears were reported by two observers separately and independently for number of micronucleated cells per 1,000 epithelial cells in oil immersion magnification and were expressed as MN score per 1,000 cells and score was compared with the spectrum of cervical lesions.
Results: The mean MN scores ± SD in NILM, inflammatory, ASC-US, LSIL, HSIL and SCC cases were 0.38±0.74, 1.04±1.09, 1.29±0.48, 1.89±0.67, 3.73±1.29, and 6.67±0.81 respectively. MN scores of SCC and HSIL were significantly high compared to NILM(p<0.001), inflammatory (p<0.001), ASCUS (p<0.001) and LSIL (p<0.001) group. LSIL showed significant difference with the normal (p<0.001), inflammatory (p=0.001), HSIL (p<0.001) and SCC (p<0.001), but not with the ASCUS(p=0.165) group.
Conclusion: MN scoring on the epithelial cells of cervix is an easy, simple, reliable, reproducible objective test done on routine Pap-stained smears and can be used as adjunct in cervical cancer screening.

Keywords:
Micronucleus, LSIL, HSIL, Squamous cell carcinoma

Doi No:-10.18231