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IP Indian Journal of Immunology and Respiratory Medicine


In vitro analysis of allergens among patients with Asthma - A clinical study


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Author Details: Girish Dandagi*,Vilol Joshi,Siddappa Dhaduti

Volume : 3

Issue : 4

Online ISSN : 2581-4222

Print ISSN : 2581-4214

Article First Page : 177

Article End Page : 182


Abstract

Background: Allergy is one of most basic immunological response which happens after exposure to substances which are safe to dominant part of individuals. On the planet 20-30% of the tenants are known to experience the ill effects of different hypersensitive issue like bronchial asthma, atopic dermatitis, unfavorably susceptible rhinitis and urticaria. From the previous 10-15 years there is a sudden radical increment in the quantity of hypersensitivities in both developed and developing nations. Urbanization, changing ways of life and expanding contamination in Indian sub-mainland has expanded the predominance of asthma. Different sorts of allergens in patients with unfavorably susceptible turmoil is a fundamental factor as a result of the clinical highlights which relies upon the nature and qualities of allergens in charge of refinement. As and when patients are getting presented to these allergens there is high limit of exacerbating the asthmatic condition. Consequently we plan to recognize such different types of allergens which we saw in a group of patients giving history of asthma.
Materials and Methods: Eighty patients giving history of asthma or recently analyzed instances of asthma were incorporated into our study group. Age ranged between 17 to 40 years. All patients with asthma were analyzed utilizing GINA 2018 rules. Patients with other foundational conditions prompting indications like asthma or windedness, history of smoking, patients on medicines were avoided from our present examination.
Before the beginning of the study ethical clearance was obtained from Institutional review board. Every one of the patients was educated about the investigation and consent was obtained. The 5ml of patient's serum was sent for in vitro examination of allergen by Immuno-EIA technique. The aggregate IgE esteem for serum more than 100KU/L is viewed as positive for sensitivity later serum IgE levels for particular allergens determined.
Results: Total of 80 patient were included in study 50 males and 30 females. Age ranged between 17 to 40years with meanage of 28.5years. Highest number of allergens noted was Housedust mite (70%) with least being chalk powder, cement dust, soap (1%) respectively.
Conclusions: Immuno-EIA remains as a one of the most rapid, easily performed, most trusted invitro technique to screen the patients with different kinds of allergens. This can help us further to manage the patient accordingly.

Keywords: Allergen; Asthma; Immuno EIA.

Doi No:-10.18231/2581-4222.2018.0044