Indian Journal of Microbiology Research


Phenotypic and Molecular detection of Mannose Resistant Haemagglutination in Escherichia coli isolates from Antenatal Women with Asymptomatic Bacteriuria


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Author Details: Prabha Poonusamy, Radhika Katragadda, Thyagarajan Ravinder

Volume : 4

Issue : 2

Online ISSN : 2394-5478

Print ISSN : 2394-546X

Article First Page : 199

Article End Page : 202


Abstract

Introduction: Asymptomatic bacteriuria is more common in pregnant females. Early screening and treatment avoids complications like acute pyelonephritis and chronic kidney failure. Escherichia coli is the most commonly encountered organism among them. Certain virulence factors associated with E.coli have a direct link in pathogenic mechanism of organism like adhesins, toxins and hemolysin.
Aim: We aimed at identifying E.coli and demonstrating phenotypic and genotypic presence of mannose resistance by hemagglutination in urinary isolates.
Materials and Method: A cross sectional study during 2014 – 2015 among pregnant females was conducted in a tertiary care hospital. About 1000 urine samples were included in study. Urine culture with antimicrobial susceptibility testing was performed as per standard microbiological conditions. Virulence factor were identified using phenotypic method i.e., haemagglutination test and subsequently confirmed by polymerase chain reaction by looking for Pap A gene expression.
Results: Out of 1000 urine samples, 118 culture positives were seen. Out 118 culture positivity, 54 (45.76%) Escherichia coli isolates causing asymptomatic bacteriuria was identified. Among the 54 isolates, phenotypic method for mannose resistance haemagglutination showed 9 positivity (16.66%), which was confirmed with PCR for Pap A expression.
Conclusion: As asymptomatic bacteriuria is more common in pregnancy, it is a must to screen pregnant females in every trimester even without any specific symptoms. Virulence factors need to be explored in order to understand the pathogenic mechanism and importance of screening the asymptomatic women.

Keywords:
Antenatal women, Asymptomatic bacteriuria, Mannose resistant hemagglutination

Doi No:-10.18231