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Indian Journal of Pharmacy and Pharmacology

Extended spectrum beta-lactamase production and biofilm formation in Klebsiella pneumoniae isolates from urinary tract samples: A tertiary care hospital experiencev

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Author Details: Amar Sunil Lobo, MS Moosabba, Rouchelle Tellis, Ronald Roche

Volume : 5

Issue : 1

Online ISSN : 2393-9087

Print ISSN : 2393-9079

Article First Page : 1

Article End Page : 3


Multidrug resistant Gram negative bacteria belonging to family Enterobacteriaceae are responsible for urinary tract infections (UTIs) that are difficult to treat. Nosocomial and community acquired UTIs are known to be existing with resistance recently. Higher drug resistance among these healthcare associated pathogenic bacteria increases the mortality, morbidity rates and the medical costs. UTIs caused by Klebsiella pneumoniae (K.pneumoniae) isolates are a major public health problem because of their multidrug-resistance to third generation cephalosporins and for their ability to produce extended spectrum beta- lactamases (ESBLs). To accesses the formation of biofilm formation and ESBL production especially in K.pneumoniae isolates from urine samples, this study has been designed in a tertiary care medical college hospital in Mangalore, Dakshina Kannada District, Karnataka, India. According to established standard methods, about 80 urine samples containing K. pneumoniae isolates were characterized and subjected for screening to antibiotic susceptibility test using Kirby Bauer disc diffusion and and presumptive ESBL production by double disk synergy test (DDST). In this study, we found that 55 (68.75%) were found to be biofilm producers. 19 (23.75%) isolates were ESBL producers and all them produced biofilm. K. pneumoniae isolates producing ESBL had a significantly greater capacity to form strong biofilm (72.4%) than non ESBL producing K. pneumoniae isolates (27.58%).

Keywords: Urinary tract infections, Klebsiella pneumoniae, Multidrug, ESBL production, Biofilm


Doi No:-10.18231/2393-9087.2018.0001