Indian Journal of Pharmacy and Pharmacology


IN VITRO STUDY OF SPONTANEOUS AND HISTAMINE INDUCED CONTRACTIONS IN PRE-ATRETIC PART EXCISED FROM A CASE OF COLONIC ATRESIA


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Author Details: Preeti Tyagi, Maloy B Mandal, A.N. Gangopadhyay

Volume : 2

Issue : 2

Online ISSN : 2393-9087

Print ISSN : 2393-9079

Article First Page : 139

Article End Page : 142


Abstract

Atresia is the congenital obstruction with complete occlusion of intestinal lumen and responsible for 95% of congenital intestinal obstructions. Colonic atresia is a rare form of intestinal atresia. Because of its rarity, limited literature is available on management and outcome of this entity. Although new operative techniques and better perioperative managements have led to an improvement, but delay in intestinal motility recovery and long term decreased motility remained common problems in postoperative period. Most of the surgeons excise the atretic part and only few centimeter of just adjacent pre-atretic part. Most of the dilated pre-atretic part is not excised usually and considered as normal but study on its exact functional status is not available. This study was done on pre-atretic part of freshly excised specimen of a case of colonic atresia in vitro to know the functionality of this part which may be helpful to decide that it should be preserve or excise. After clearing of fecal matter, 2-3 millimeter wide and 15-20 millimeter long longitudinal strip was prepared and mounted in organ bath filled with Krebs Ringer solution continuously bubbled with 100% oxygen. Spontaneous contractions were observed with the frequency of approximately 3 peaks/ hour and amplitude of 27.23 gram per gram wet tissue. There was no response by lowest dose of histamine (0.1 μM) while dose dependent increase in the contractile force by different cumulative concentrations of histamine (1, 10 & 100 μM) was observed. It can be concluded that pre-atretic part is normal up to some extent especially in respect to the pacemaker activity and histaminergic pathway so it should be preserved. However, detailed functional and histopathological study on more number of cases as well as on control specimens by using different agonists and their blockers are needed.


Key words: Colonic atresia, Contractions, Histamine, In vitro study

Doi No:-10.18231