COVID-19 Update - This is to inform you that the Government of India has announced a complete lockdown in India 22nd March 2020 to 3rd May 2020. As a result, our offices will now be closed till 3rd May 2020 and all our employees will be working from home. Office telephones will not be answered, and therefore you are requested to direct all your queries related to manuscript submission, review process, publication etc. at below mentioned details. editor@innovativepublication.com, rakesh.its@gmail.com, Mob. 8826373757, 8826859373, 9910947804


Print ISSN:-2581-5725

Online ISSN:-2456-9267

CODEN : IACHCL

Current Issue

Year 2020

Volume: 5 , Issue: 1

Article Access statistics

Viewed: 528

Emailed: 0

PDF Downloaded: 151

IP Archives of Cytology and Histopathology Research


Microwave assisted silver stain for pap smear to improve the identification of coccobacillary flora


Full Text PDF Share on Facebook Share on Twitter


Author Details : M M Kamal, Kanica Jha*, Hirakani Mankar

Volume : 4, Issue : 1, Year : 2019

Article Page : 31-35


Suggest article by email

Abstract

Introduction: Pap smear has been utilized since a long time in detecting cervical pre-cancers and cancers. Their potential in diagnosing pathogenic organisms however is underestimated. Reporting organisms morphologically consistent with Candida species, in cervicovaginal smears, is easier, but coexisting coccobacillary flora is often missed in the bargain. Such co-existing infections make the treatment difficult for clinicians, prolonging the distressing symptoms of patients. We evaluated modified silver staining technique using a microwave - JONES MARRES STAIN (JM) and tried comparing the results with conventional Pap smears to find any additional flora.
Aim: To identify co-existing organisms in addition to Candida in a Pap smear stained with Jones Marres stain.
Materials and methods: This retrospective prevalence study that included 82 consecutive conventional Pap smears routinely stained with Pap stain was then subjected to silver stain in a microwave.

Inclusion criteria: Pap smears reported as showing Candida group of organisms.
Results: In the 82 smears evaluated, JM stain helped in easier picking up of additional coccobacillary flora in 37(45%) of cases in comparison to 4(4.87%).These coexisting infections were missed in conventional Pap smear reporting.
Conclusion: Modified JM stain helped in diagnosing additional flora thus reducing the chances of missing out co-infections and hence is useful for providing effective treatment

Keywords: Microwave Silver stain, Co-infection, Pap smear, Coccobacillary flora.

Doi : 10.18231/2456-9267.2019.0005

How to cite : Kamal M M, Jha K, Mankar H , Microwave assisted silver stain for pap smear to improve the identification of coccobacillary flora. IP Arch Cytol Histopathol Res 2019;4(1):31-35

Copyright © 2019 by author(s) and IP Arch Cytol Histopathol Res. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (CC BY 4.0) (creativecommons.org)