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International Journal of Clinical Biochemistry and Research

A pilot study to compare urinary protein of fractional urine collected over 24h period versus traditional 24h urine collection method

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Research Article

Author Details : Ravi Prakash Deshpande, Anitha Devanath, Limesh Marisiddappa, Girish Konasagara Shanthaveeranna*

Volume : 7, Issue : 1, Year : 2020

Article Page : 80-86

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Introduction: 24 hours traditional urine collection method is cumbersome and tedious process with less
patient compliance. Our study proposes a novel fractional urine collection method for protein estimation
that would entail collection of small volumes of urine each time patient voids Over 24h Period. Our aim
was to compare urine protein in proposed method versus traditional 24hour sampling method.
Materials and Methods: Cross-sectional, prospective study was a collaborative work between
Departments of Nephrology and Biochemistry in our tertiary care hospital. Volunteers (48) and Chronic
Kidney disease subjects (52) were recruited. Out of which, 76 subjects were selected. Novel method
was compared with conventional method in each of these groups, before and after centrifugation and with
and without preservative. Intraclass correlation coefficient (ICC) and Bland Altman analysis was used to
evaluate for agreement between the two methods.
Results: All the values were combined without categorization into subgroups. Fractional urine collection
method (before centrifugation) without preservative (F1) and with preservative (F2) was compared to
conventional method, ICC was 0.93 (C.I, 0.9 to 0.95) and 0.96 (C.I, 0.94 to 0.97) respectively. After
centrifugation, fractional urine collection method for F1and F2 versus conventional method, ICC was 0.95
(C.I, 0.92 to 0.97) and 0.91 (C.I, 0.86 to 0.94) respectively.
Conclusions: The novel collection method is comparable and reliable as traditional 24-hour method. The
use of thymol as preservative leads to negative interference and centrifugation is mandatory before urine
analysis. With proper sampling, urine protein by novel method has a good agreement with the conventional

Keywords: Factional, Urine protein, Traditional 24h urine, Urine creatinine.

Doi : 10.18231/j.ijcbr.2020.017

How to cite : Deshpande R P, Devanath A, Marisiddappa L, Shanthaveeranna G K, A pilot study to compare urinary protein of fractional urine collected over 24h period versus traditional 24h urine collection method. Int J Clin Biochem Res 2020;7(1):80-86

Copyright © 2020 by author(s) and Int J Clin Biochem Res. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (CC BY 4.0) (