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International Journal of Clinical Biochemistry and Research


A prospective comparative study of glucose estimation by hexokinase and glucose oxidase-peroxidase methods


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Research Article

Author Details : K Maria Kumar*, K Vijayakumari, K Sri Lakshmi

Volume : 6, Issue : 3, Year : 2020

Article Page : 356-362


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Abstract

Aims and Objectives: Error analysis of the comparative study of glucose estimation with the comparison
and regression analysis.
Introduction: Glucose is the major carbohydrate found in the blood and a chief source of energy in human
body. Estimation of glucose is very important in clinical diagnosis of hyperglycemia, hypoglycemia and
normoglycemia. Accuracy of test results are very important, requires considerable effort and cost. For
introducing in the laboratory the test method has to be evaluated by the process called quality control.
Method selection and evaluation is important in improving efficiency.
Materials and Methods: The preferred sample for Glucose assay is fasting serum or plasma. A total of
105 samples are analysed for the quantitative estimation of blood glucose by the reference method.
Discussion and Results: In the present study comparison between a test method (Glucose oxidaseperoxidase)
and a reference method (Hexokinase), the errors are less than TEa. Mean value of Hexokinase
method is 311.36 137.315 and mean value Glucose oxidase-peroxidase method is 291.87 133.412.
p-value on comparison = 0.00014. With this the test method can be an alternative to reference method in
terms of accuracy and precision and analytical sensitivity and specificity.
Conclusion: Glucose oxidase-peroxidase method is also a suitable method for the quantitative estimation
of glucose in need of clinical requirements.

Keywords: Serum glucose, Comparison and correlation, Glucose oxidase -peroxidase, Hexokinase, Method evaluation, Quality control.

Doi : 10.18231/j.ijcbr.2019.078

How to cite : Kumar K M, Vijayakumari K, Lakshmi K S, A prospective comparative study of glucose estimation by hexokinase and glucose oxidase-peroxidase methods. Int J Clin Biochem Res 2020;6(3):356-362

Copyright © 2020 by author(s) and Int J Clin Biochem Res. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (CC BY 4.0) (creativecommons.org)