COVID-19 Update - This is to inform you that the Government of India has announced a complete lockdown in India 22nd March 2020 to 3rd May 2020. As a result, our offices will now be closed till 3rd May 2020 and all our employees will be working from home. Office telephones will not be answered, and therefore you are requested to direct all your queries related to manuscript submission, review process, publication etc. at below mentioned details.,, Mob. 8826373757, 8826859373, 9910947804

Article Access statistics

Viewed: 766

Emailed: 0

PDF Downloaded: 198

IP Indian Journal of Conservative and Endodontics

Comparative evaluation of antimicrobial efficacy of MTAD, DMSA (Di Mercaptosuccinic acid), sodium hypochlorite (NaOCl) and chlorhexidine against E. faecalis: An ex-vivo study

Full Text PDF Share on Facebook Share on Twitter

Author Details : Vivek Kapoor, Ruchi Singla, Rajni Kapoor

Volume : 2, Issue : 4, Year : 2017

Article Page : 128-132

Suggest article by email


Aim: The aim of this study was to compare the anti-microbial efficacy of four root canal irrigants, MTAD, 10% Di Mercaptosuccinic acid (DMSA), 5.25% Sodium Hypochlorite and 2% Chlorhexidine gluconate against Enterococcus Faecalis.
Materials & Methods: 75 extracted single rooted teeth with single canal were selected for this study. Conventional access preparations were made and the canals were instrumented 1 mm beyond the apical foramen with K-files up to size 50. The teeth were autoclaved. Pure culture of E. Faecalis was grown in brain heart infusion broth and was set to 4 Mac Farland’s standard. 5 micro-litre of the broth was used to infect each of the root canal. The teeth were then divided into 5 groups (n=15) according to the irrigant delivered; group 1 was irrigated with MTAD, group 2 with 10% DMSA, group 3 with 5.25% Sodium Hypochlorite, group 4 with 2% Chlorhexidine gluconate and group 5 was irrigated with Normal saline (control group). Samples were collected from the root canals, after 5 minutes and 48 hours of irrigation, with help of absorbent paper points and were immediately transferred to test tubes containing brain heart infusion broth and incubated. Occurrence of broth turbidity was indicative of the viable bacteria remaining in the root canal. The test tubes where turbidity occurred were inoculated onto chocolate Agar plates to check for E. Faecalis. Results were analyzed statistically using Chi-Square test and Mann-Whitney test (p<0.05).
Results: Viable bacteria were found in all the canals of the control and 10% DMSA group. No viable bacteria were remaining in canals after 5 min of irrigation with MTAD and CHX whereas 67% of the root canals irrigated with NaOCl showed growth. After 48 hours of irrigation only teeth irrigated with CHX were free from viable bacteria.

Conclusion: 2% Chlorhexidine has a superior anti-microbial and residual anti-microbial activity against E. Faecalis compared to all the irrigants tested.

MTAD; DMSA, Chlorhexidine, Sodium Hypochlorite, E. Faecalis.

How to cite : Kapoor V, Singla R, Kapoor R, Comparative evaluation of antimicrobial efficacy of MTAD, DMSA (Di Mercaptosuccinic acid), sodium hypochlorite (NaOCl) and chlorhexidine against E. faecalis: An ex-vivo study. IP Indian J Conserv Endod 2017;2(4):128-132

Copyright © 2017 by author(s) and IP Indian J Conserv Endod. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (CC-BY-NC 4.0) (