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Indian Journal of Clinical and Experimental Ophthalmology

Agranulocyte & neutrophil nucleus enhancement through 510nm wavelength of light

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Research Article

Author Details : Nikita Shivhare Mitra*, Saksha Shinde, Neeraj Sharma, Chandra K ant Singh Tekam, Mansi Maurya

Volume : 6, Issue : 2, Year : 2020

Article Page : 279-281

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Enhanced features can be easily segmented and used for classification algorithms. Light wavelength of
510nm is used as illumination source for bright field microscope. This specific wavelength of light enhances
nucleus, granules in White blood Cells (WBCs). Eosinophil and basophil has coarse granules which also
get enhanced hence nucleus segmentation gets difficult. This method is effective in enhancing nucleus of
agranulocytes viz. Lymphocyte, Monocyte and Neutrophil of normal person. This modification in image
acquisition of pathological images will further help in quick segmentation of features.

Keywords: Bright field microscope, Image enhancement, 510nm wavelength of green light, LED illumination, WBC nucleus.

Doi : 10.18231/j.ijceo.2020.060

How to cite : Mitra N S, Shinde S , Sharma N , Tekam C K A S, Maurya M , Agranulocyte & neutrophil nucleus enhancement through 510nm wavelength of light. Indian J Clin Exp Ophthalmol 2020;6(2):279-281

Copyright © 2020 by author(s) and Indian J Clin Exp Ophthalmol. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (CC-BY-NC 4.0) (