COVID-19 Update - This is to inform you that the Government of India has announced a complete lockdown in India 22nd March 2020 to 3rd May 2020. As a result, our offices will now be closed till 3rd May 2020 and all our employees will be working from home. Office telephones will not be answered, and therefore you are requested to direct all your queries related to manuscript submission, review process, publication etc. at below mentioned details.,, Mob. 8826373757, 8826859373, 9910947804

Print ISSN:-2394-546X

Online ISSN:-2394-5478


Current Issue

Year 2020

Volume: 7 , Issue: 1

Article Access statistics

Viewed: 406

Emailed: 0

PDF Downloaded: 198

Indian Journal of Microbiology Research

Antibiogram of staphylococcal isolates with special reference to inducible clindamycin resistance

Full Text PDF Share on Facebook Share on Twitter

Research Article

Author Details : S Tanmaya, T A Dhanalakshmi*, D Venkatesha

Volume : 6, Issue : 4, Year : 2019

Article Page : 323-327

Suggest article by email


Introduction and Objectives: Expression of inducible clindamycin resistance (iMLSB) by
Staphylococcal isolates limits the effective use of clindamycin resulting in treatment failure. The present
study was aimed to estimate the prevalence of iMLSB.
Materials and Methods: Fifty Staphylococci isolated from various clinical specimens were identified by
standard microbiological methods and were subjected to antibiotic susceptibility testing by Kirby-Bauer
disc diffusion method and D test. Methicillin resistance was detected by cefoxitin disc diffusion method.
Results: Staphylococcus aureus constituted 62% and co-agulase negative Staphylococci 38% of total
Staphylococcal isolates. Methicillin resistance was noted in 27(54%) of isolates. Seventeen (34%) isolates
were sensitive to both erythromycin and clindamycin. Among the resistant phenotypes, constitutive
clindamycin resistance (cMLSB), Macrolide Streptogramin B resistance (MS) phenotype and iMLSB
was found in 16 (32%), 10 (20%) and in 7(14%) isolates respectively. Majority of the Staphylococcal
isolates showed resistance to penicillin (93.6%) followed by ciprofloxacin (79.6%) and erythromycin
(66%). Linezolid resistance was observed in 8% of isolates. None of the isolates were found resistant
to vancomycin.
Interpretation and Conclusion: Routine testing for iMLSB for all clinical isolates of Staphylococci
should be carried out to prevent failure of clindamycin therapy. Isolation of linezolid resistant
Staphylococci is an alarming sign and a matter of concern.

Keywords: D test, Inducible clindamycin resistance, Methicillin resistance, Staphylococci.

Doi : 10.18231/j.ijmr.2019.069

How to cite : Tanmaya S, Dhanalakshmi T A, Venkatesha D, Antibiogram of staphylococcal isolates with special reference to inducible clindamycin resistance. Indian J Microbiol Res 2019;6(4):323-327

Copyright © 2019 by author(s) and Indian J Microbiol Res. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (CC-BY-NC 4.0) (