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Indian Journal of Microbiology Research


Purification and characterization of bioactive secondary metabolites isolated from soil bacteria


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Author Details : Amit Pandey, Ankita Srivastava, Niharika Chandra, Dheerendra Kumar, Sunil Kumar

Volume : 5, Issue : 2, Year : 2018

Article Page : 188-193


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Abstract

The search for new bioactive compounds from the natural environment has rapidly been gaining momentum with the increase in multi-drug resistant (MDR) pathogens. In the present study, the antimicrobial potential of new bacterial species has been evaluated by initial screening of fourteen soil samples. Primary and secondary screening was performed against Escherichia coli, Staphylococcus aureus and Pseudomonas aeruginosa. Four active isolates were selected for further study. Microbial strains were identified on the basis of growth conditions and other biochemical characters. One most active microbial strain was identified using 16S rRNA sequence homology and designated as Bacillus cereus (C13). Furthermore the production of secondary metabolites was done in optimized production media and the analysis involved HPLC method to identify metabolites based on retention time where 2.833 min retention time obtained using C18 column at 265 nm wavelength, which is similar to valinomycin antibiotic which is also secreted by Streptomyces griseus. It shows presence of bioactive secondary metabolites which is responsible for antibacterial activity of bacterial isolates.

Keywords: Antimicrobial activity, Actinomycetes, Methanolic extract, Minimum inhibitory concentration, Streptomyces

Doi : 10.18231/2394-5478.2018.0040

How to cite : Pandey A, Srivastava A, Chandra N, Kumar D, Kumar S, Purification and characterization of bioactive secondary metabolites isolated from soil bacteria. Indian J Microbiol Res 2018;5(2):188-193

Copyright © 2018 by author(s) and Indian J Microbiol Res. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (CC BY 4.0) (creativecommons.org)