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Indian Journal of Microbiology Research


Correlation of VITEK -2 and conventional tests in identification of Non Fermenting Gram Negative Bacilli in a Tertiary Care Hospital


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Author Details : Shweta Sharma, R K Agarwal, Barnali Kakati

Volume : 5, Issue : 2, Year : 2018

Article Page : 198-201


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Abstract

Introduction: The non-fermentative Gram negative bacilli (NFGNB) are a group of aerobic, non-spore forming, Gram negative bacilli that either do not utilize carbohydrates as a source of energy or degrade them through metabolic pathways other than the fermentation.
Material and Methods:The study was undertaken to identify various non- fermenters isolated from patients in our hospital, a Tertiary Care Center at Uttarakhand. 252 (14.15%) non-fermenters were isolated from 1780 clinical specimens on MacConkey and blood agar plates incubated at 35°C for 24-48 hours. Any growth that was identified as non-fermenters by Vitek-2 was subjected to identification by additional conventional tests.
Results and Conclusion: Patients mostly belonged to age group of 51 to 70 years and were commonly male. Sputum & ET secretion were the commonest samples. Psuedomonas aeruginosa was the commonest isolate followed by Acinetobacter calcoacetecus baumanii complex. Out of total 252 isolates, 221(87.69%) could be accurately identified upto species level by conventional tests. The conventional tests could identify 243 (96.42%) isolates upto genus level. Majority of them showed multidrug resistance. The most effective antibiotics were Tigecycline and Polymyxin B/ Colistin.

Keywords: Non- fermenters, VITEK -2, Additional conventional biochemical tests

Doi : 10.18231/2394-5478.2018.0042

How to cite : Sharma S, Agarwal R K, Kakati B, Correlation of VITEK -2 and conventional tests in identification of Non Fermenting Gram Negative Bacilli in a Tertiary Care Hospital. Indian J Microbiol Res 2018;5(2):198-201

Copyright © 2018 by author(s) and Indian J Microbiol Res. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (CC BY 4.0) (creativecommons.org)