COVID-19 Update - This is to inform you that the Government of India has announced a complete lockdown in India 22nd March 2020 to 14th April 2020. As a result, our offices will now be closed till 14th April 2020 and all our employees will be working from home. Office telephones will not be answered, and therefore you are requested to direct all your queries related to manuscript submission, review process, publication etc. at below mentioned details.,, Mob. 8826373757, 8826859373, 9910947804

Article view: 433

Article download: 209

Indian Journal of Microbiology Research

Microbiological spectrum and antimicrobial susceptibility patterns of various isolates from endotracheal tube aspirates in a tertiary care hospital, Hyderabad, Telangana

Full Text PDF Share on Facebook Share on Twitter

Author Details : A. Swati, K. Yamini, R. V. Rajkumar

Volume : 5, Issue : 2, Year : 2018

Article Page : 202-207

Suggest article by email


Introduction: Endotracheal aspirates ( ET) are relatively simple ,easy to collect at the bedside and are used to evaluate the causes of lower respiratory tract infections in ventilated patients.Several studies reported multidrug resistant bacteria like Klebsiella, Pseudomonas and Acinetobacter spp,gram positive bacteria like S.aureus, S.pneumoniae ,Coagulase negative staphylococcus (CONS) and fungi like Candida spp in ET cultures. Even though Quantitation of the bacteria isolated from ET aspirates has been proposed as a criterion to distinguish colonization from true infection, only few studies have been done .The main aim of the present study was to evaluate the microbiological spectrum and antimicrobial susceptibility pattern of isolates from ET aspirates.
Materials and Methods:ET aspirates were processed employing quantitative cultures. Growth of ?105cfu/ml was considered to be significant.Organism Identification and Antimicrobial susceptibility testing was performed according to standard guidelines. The gram negative isolates were further tested for ESBL/carbapenamase production and gram positive as methicillin sensitive / methicillin resistance.
Results:Culture positivity was 67%. Acinetobacter spp was the most common isolate (46%) followed by Klebsiella spp (26%) and Pseudomonas spp (17%), E.coli (5%), S.aureus (2%), CONS (2%) and Candida (2%). Acinetobacter spp were extremely drug resistant. ESBL was produced by 38% and 64% of E. coli and Klebsiella spp.

Out of the 15, 40% of the Pseudomonas spp and of 127 isolates, 63.7% of Acinetobacter spp were positive for carbapenamase production respectively .Out of the 7, 6 (86%) were methicillin resistant Staphylococcus aureus.5 (83%) of CONS (n=6) were methicillin resistant.
Conclusion:We conclude that ET microscopic examination and quantitative cultures can be supportive in earlier prediction of ventilator-associated pneumonia.

Keywords: Endotracheal aspirates, Microbiological spectrum, Quantitative cultures, Multidrug resistant organisms, infection control

Doi : 10.18231/2394-5478.2018.0043

How to cite : Swati A, Yamini K, Rajkumar R V, Microbiological spectrum and antimicrobial susceptibility patterns of various isolates from endotracheal tube aspirates in a tertiary care hospital, Hyderabad, Telangana. Indian J Microbiol Res 2018;5(2):202-207

Copyright © 2018 by author(s) and Indian J Microbiol Res. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (CC BY 4.0) (