COVID-19 Update - This is to inform you that the Government of India has announced a complete lockdown in India 22nd March 2020 to 3rd May 2020. As a result, our offices will now be closed till 3rd May 2020 and all our employees will be working from home. Office telephones will not be answered, and therefore you are requested to direct all your queries related to manuscript submission, review process, publication etc. at below mentioned details.,, Mob. 8826373757, 8826859373, 9910947804

Print ISSN:-2394-2789

Online ISSN:-2394-2797


Current Issue

Year 2020

Volume: 7 , Issue: 1

Article Access statistics

Viewed: 282

Emailed: 0

PDF Downloaded: 105

International Journal of Pharmaceutical Chemistry and Analysis

Formulation and characterization of cyclophosphamide injections using lyophilization technique

Full Text PDF Share on Facebook Share on Twitter

Research Article

Author Details : D MK. Chakradhar, A Sunitha, B Sofia, Grandhi Srikar*

Volume : 6, Issue : 3, Year : 2019

Article Page : 69-75

Suggest article by email


Introduction: The parenteral route of administration is generally adopted for rapid onset of action and 100% bioavailability especially for the drugs which have poor bioavailability through peroral route. Newly developed drugs often show poor solubility and require novel dosage forms such as liposomes, nanoparticles to minimize solubility problems and side effects due to toxicity. These dosage forms are often inherently labile due to agglomeration, sedimentation etc., and can be stabilized and manufactured by freeze drying. Lyophilization is the most common method for manufacturing solid pharmaceuticals products and is central to the preservation of materials which must be dried thoroughly in order to ensure stability and require a gentle, easily sterilizable process.
Materials and Methods: The current research work involves the formulation of cyclophosphamide injections using lyophilization technique by using different concentrations of cyclophosphamide. The formulated cyclophosphamide injections are further subjected to different evaluation tests such as melting point, differential scanning calorimetry, dissolution rate study, solution stability, pH stability, rubber stopper compatibility and photostability. 
Results: The moisture content of the formulation is greatly reduced to as low as 0.9% (w/w) thus enhancing the stability of the product.
Conclusion: The lyophilized technique proved to be an advantage for the development of stable injectable dosage form of Cyclophosphamide.

Keywords: Lyohilization, Stability, Sterilized, cyclophosphamide, Mannitol.

Doi : 10.18231/j.ijpca.2019.014

How to cite : Chakradhar D M, Sunitha A, Sofia B, Srikar G, Formulation and characterization of cyclophosphamide injections using lyophilization technique. Int J Pharm Chem Anal 2019;6(3):69-75

Copyright © 2019 by author(s) and Int J Pharm Chem Anal. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (CC-BY-NC 4.0) (