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International Journal of Pharmaceutical Chemistry and Analysis


Area under Curve Method Development and Validation of Midodrine Hydrocholride


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Author Details : Pritam S. Jain, Prashant V. Bhadane, Harshal P. Chaudhari, Sanjay J Surana

Volume : 2, Issue : 4, Year : 2015

Article Page : 154-160


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Abstract

A simple, rapid, accurate and economical Area under Curve method has been developed for estimation of Midodrine hydrochloride from bulk and pharmaceutical formulation. The λ max of Midodrine hydrochloride in water was found to be 289 nm. The drug follows linearity in the concentration range 12-84 μg/ml with correlation coefficient value 0.999. The proposed method was applied to pharmaceutical formulation and % amount of drug estimated 97.00% – 101.00% was found in good agreement with the label claim. The accuracy of the method was checked by recovery experiment performed at three different levels i.e., 80%, 100% and 120%. The % recovery was found to be in the range 97.00% – 101.00%. The low values of % RSD are indicative of the accuracy and reproducibility of the method. The precision of the method was studied as an intra-day, inter-day variations and repeatability. The % R.S.D. value less than 2 indicate that the method was precise. Ruggedness of the proposed method was studied with the help of two analysts. The above method was a rapid and cost-effective quality-control tool for routine analysis of Rizatriptan benzoate in bulk and in pharmaceutical dosage form.

Keywords: Midodrine hydrochloride, UV-Spectrophotometry, Area under Curve, Validation

 

How to cite : Jain P S, Bhadane P V, Chaudhari H P, Surana S J, Area under Curve Method Development and Validation of Midodrine Hydrocholride. Int J Pharm Chem Anal 2015;2(4):154-160

Copyright © 2015 by author(s) and Int J Pharm Chem Anal. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (CC BY 4.0) (creativecommons.org)