COVID-19 Update - This is to inform you that the Government of India has announced a complete lockdown in India 22nd March 2020 to 3rd May 2020. As a result, our offices will now be closed till 3rd May 2020 and all our employees will be working from home. Office telephones will not be answered, and therefore you are requested to direct all your queries related to manuscript submission, review process, publication etc. at below mentioned details.,, Mob. 8826373757, 8826859373, 9910947804

International Journal of Pharmaceutical Chemistry and Analysis

Area under Curve Method Development and Validation of Midodrine Hydrocholride

Full Text PDF

Author Details: Pritam S. Jain, Prashant V. Bhadane, Harshal P. Chaudhari, Sanjay J Surana

Volume : 2

Issue : 4

Online ISSN : 2394-2797

Print ISSN : 2394-2789

Article First Page : 154

Article End Page : 160


A simple, rapid, accurate and economical Area under Curve method has been developed for estimation of Midodrine hydrochloride from bulk and pharmaceutical formulation. The λ max of Midodrine hydrochloride in water was found to be 289 nm. The drug follows linearity in the concentration range 12-84 μg/ml with correlation coefficient value 0.999. The proposed method was applied to pharmaceutical formulation and % amount of drug estimated 97.00% – 101.00% was found in good agreement with the label claim. The accuracy of the method was checked by recovery experiment performed at three different levels i.e., 80%, 100% and 120%. The % recovery was found to be in the range 97.00% – 101.00%. The low values of % RSD are indicative of the accuracy and reproducibility of the method. The precision of the method was studied as an intra-day, inter-day variations and repeatability. The % R.S.D. value less than 2 indicate that the method was precise. Ruggedness of the proposed method was studied with the help of two analysts. The above method was a rapid and cost-effective quality-control tool for routine analysis of Rizatriptan benzoate in bulk and in pharmaceutical dosage form.

Keywords: Midodrine hydrochloride, UV-Spectrophotometry, Area under Curve, Validation


Doi No:-10.18231