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Indian Journal of Pathology and Oncology

EDTA dependent pseudothrombocytopenia - cause and incidence

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Author Details : Ujjwala V. Tulluri, Yash R. Kale, SR Agashe, PP Patil

Volume : 3, Issue : 4, Year : 2016

Article Page : 701-703

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 Aims and Objectives:

1.        To find out the incidence of EDTA dependent pseudothrombocytopenia(PTCP)

2.        To confirm EDTA dependent pseudothrombocytopaenia(PTCP) by counting the platelets using anticoagulants other than EDTA (citrate and heparin)

Introduction: Pseudothrombocytopenia (PTCP) meaning there by a spuriously low platelet count.
EDTA induced clumping of platelets is one of the important cause of pseudothrombocytopaenia.
Materials and Method

Inclusion criteria: All 3000 blood samples received during study period in hematology laboratory of BVDU Medical college sangli for complete blood count (CBC) or platelet count

Exclusion criteria:

1.        Patients with known cause of thrombocytopenia were excluded.

2.        Thrombocytopenia due to technical errors.

Result: The incidence of EDTA Dependent Pseudothrombocytopenia was 0.13%.
Conclusion: The incidence of EDTA dependent Pseudothrombocytopenia is quite low.
Every sample with a low platelet count with EDTA as anticoagulant and/or presence of platelet clumps on the peripheral blood smear, and after ruling out other causes of pseudothrombocytopenia, must be collected again in a vaccutainer containing different anticoagulant (heparin and citrate). If the platelet count rises therein, it should be considered as a case of EDTA dependent Pseudothrombocytopenia.

: Platelet count, Pseudothrombocytopenia, EDTA dependent Pseudothrombocytopenia

How to cite : Tulluri U V, Kale Y R, Agashe S, Patil P, EDTA dependent pseudothrombocytopenia - cause and incidence. Indian J Pathol Oncol 2016;3(4):701-703

Copyright © 2016 by author(s) and Indian J Pathol Oncol. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (CC-BY-NC 4.0) (