COVID-19 Update - This is to inform you that the Government of India has announced a complete lockdown in India 22nd March 2020 to 14th April 2020. As a result, our offices will now be closed till 14th April 2020 and all our employees will be working from home. Office telephones will not be answered, and therefore you are requested to direct all your queries related to manuscript submission, review process, publication etc. at below mentioned details. editor@innovativepublication.com, rakesh.its@gmail.com, Mob. 8826373757, 8826859373, 9910947804

Article view: 486

Article download: 204

Indian Journal of Pathology and Oncology


Utility of C-Kit Expression by Immunohistochemistry as a Prognostic and Theranostic Marker in Colorectal Carcinoma


Full Text PDF Share on Facebook Share on Twitter


Author Details : G. Barathi, Leena D. Joseph, S. Rajendiran, Suresh Varadarajan

Volume : 4, Issue : 3, Year : 2017

Article Page : 385-390


Suggest article by email


Abstract

Objectives: Targeted therapy is now available for various carcinomas with growth factor receptor mutations. C-Kit, a tyrosine kinase receptor is overexpressed in many epithelial malignancies. The aim of this study is to determine the role of c-Kit (CD117) expression by Immunohistochemistry (IHC) in colorectal carcinoma (CRC) and to correlate the same with tumor grade, stage, nodal status and metastasis.
Methods: This is a retrospective study conducted on paraffin blocks of histopathology proven CRC. Monoclonal antibody detected against c-kit antigen was observed in the cytoplasm and epithelial membrane of the tumour cells and c-Kit expression was given a score based on staining intensity and percentage of cells showing positivity. A statistical analysis using chi square test was performed to find any difference between the various tumor grade, stage, nodal status and metastasis with respect to their c-kit expression by IHC.
Results: c-Kit was negative in 55% of the cases and out of 45% positive cases 3+ positivity was noted in 5% of the cases only. 2+ positivity was seen in 9% and 1+ in 31%. Statistical data showed a p-value of 0.96, 0.70, 0.48 and 0.68 for grade, stage, nodal status and metastasis of the tumor with respect to c-kit expression respectively.
Conclusion: C-kit expression by IHC has only a limited role and is statistically not significant as a prognostic or theranostic marker in CRC. Molecular analysis of the tyrosine kinase receptor mutation is needed to determine its aberrant expression.

Keywords:
c-Kit, Colon cancer, Targeted therapy

How to cite : Barathi G, Joseph L D, Rajendiran S, Varadarajan S, Utility of C-Kit Expression by Immunohistochemistry as a Prognostic and Theranostic Marker in Colorectal Carcinoma. Indian J Pathol Oncol 2017;4(3):385-390

Copyright © 2017 by author(s) and Indian J Pathol Oncol. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (CC BY 4.0) (creativecommons.org)