COVID-19 Update - This is to inform you that the Government of India has announced a complete lockdown in India 22nd March 2020 to 14th April 2020. As a result, our offices will now be closed till 14th April 2020 and all our employees will be working from home. Office telephones will not be answered, and therefore you are requested to direct all your queries related to manuscript submission, review process, publication etc. at below mentioned details. editor@innovativepublication.com, rakesh.its@gmail.com, Mob. 8826373757, 8826859373, 9910947804

Article view: 209

Article download: 129

Indian Journal of Pharmacy and Pharmacology


Reverse phase high performance liquid chromatography and UV-Visible method for the simultaneous estimation of gemifloxacin


Full Text PDF Share on Facebook Share on Twitter


Research Article

Author Details : Senthilraja M*

Volume : 6, Issue : 2, Year : 2019

Article Page : 60-64


Suggest article by email


Abstract

A novel UV-Visible and reversed phase High Performance Liquid Chromatography method has been developed and validated for the simultaneous analysis of gemifloxacin and their formulation. The UV-Visible method was carried with folin reagent and reverse Phase HPLC method with acetonitrile: phosphate buffer (40:60%v/v) mobile phase, having a pH of 3.0 at a wavelength of 272 nanometer. The linearity range starts from 2 to10?g/ml and 1 to 5?g/ml, percentage recovery was found to be 98.55% and 99.68%, limit of detection 0.2651?g and 0.1588?g and 0.8033?g and 0.4811?g for the UV-Visible and RP-HPLC methods respectively.

Keywords: Reversed phase-HPLC, UV-Visible, Gemifloxacin, Folin-Ciocalteu phenol reagent, Phosphate buffer.

Doi : 10.18231/j.ijpp.2019.014

How to cite : Senthilraja M, Reverse phase high performance liquid chromatography and UV-Visible method for the simultaneous estimation of gemifloxacin. Indian J Pharm Pharmacol 2019;6(2):60-64

Copyright © 2019 by author(s) and Indian J Pharm Pharmacol. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (CC BY 4.0) (creativecommons.org)