COVID-19 Update - This is to inform you that the Government of India has announced a complete lockdown in India 22nd March 2020 to 3rd May 2020. As a result, our offices will now be closed till 3rd May 2020 and all our employees will be working from home. Office telephones will not be answered, and therefore you are requested to direct all your queries related to manuscript submission, review process, publication etc. at below mentioned details. editor@innovativepublication.com, rakesh.its@gmail.com, Mob. 8826373757, 8826859373, 9910947804

Article Access statistics

Viewed: 641

Emailed: 0

PDF Downloaded: 179

Journal of Pharmaceutical and Biological Sciences


Comparative analysis of efficiency of pore size and materials of filters for the isolation and purification of leptospires


Full Text PDF Share on Facebook Share on Twitter


Author Details : Prabhusaran Nagarajan, Natarajaseenivasan Kalimuthusamy, Joseph Pushpa Innocent Danialas

Volume : 5, Issue : 5, Year : 2017

Article Page : 199-203


Suggest article by email

Abstract

Filtration is a major procedure performing in the culture media preparation and avoiding contamination during sub culturing of leptospiral strains. The main objective of this study is to determine the effective filter material and pore size by compare among various filters. The filter materials evaluated in this study are glass fiber (1μm pore diameter), millipore polyvinylidene fluoride (0.22μm and 0.45μm) nitrocellulose (0.22 and 0.45μm pore diameters) and nylon mesh (37μm). The leptospiral cultures in aqueous was filtered by using all filters for the determination of effective filtration of leptospires. All the filtrates were examined by dark field microscopy and the effective filters were selected for further analysis. The filtrate also inoculated in bacterial and fungal culture media to find out any contamination found. Our results suggested that the isolation of 100% leptospires using millipore polyvinylidene fluoride and the optimal pore diameter should be 0.45μm from EMJH broth with well cultured leptospires. Although filtration method can be applicable for isolation and purification of leptospires, it is unclear whether this is a useful method in all situations. This study concluded with the statement “large proportion of leptospires can be filtered by membrane filter at the pore size of 0.45μm”.

Keywords:
Leptospira, filtration technique, pore size, EMJH broth

How to cite : Nagarajan P, Kalimuthusamy N, Danialas J P I, Comparative analysis of efficiency of pore size and materials of filters for the isolation and purification of leptospires. J Pharm Biol Sci 2017;5(5):199-203

Copyright © 2017 by author(s) and J Pharm Biol Sci. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (CC-BY-NC 4.0) (creativecommons.org)