Calcium Hydroxide has for long been the gold standard for Vital Pulp Therapy. Thus the aim of this study was to comparatively evaluate the effect of Biodentin™ Vs. Calcium hydroxide on exposed human pulp. The effect of the materials on Maxillary human premolars destined for extraction and after histological analysis it was seen that the quality and quantity of calcific barrier formation with Biodentin™ was superior to that of Calcium hydroxide.